A framework for human microbiome research.

TitleA framework for human microbiome research.
Publication TypeJournal Article
Year of Publication2012
Corporate AuthorsConsortium, HMicrobiome
JournalNature
Volume486
Issue7402
Pagination215-21
Date Published2012 Jun 14
ISSN1476-4687
KeywordsAdolescent, Adult, Bacteria, Female, Humans, Male, Metagenome, Metagenomics, Reference Standards, RNA, Ribosomal, 16S, Statistics as Topic, Young Adult
Abstract

A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a population-scale framework to develop metagenomic protocols, resulting in a broad range of quality-controlled resources and data including standardized methods for creating, processing and interpreting distinct types of high-throughput metagenomic data available to the scientific community. Here we present resources from a population of 242 healthy adults sampled at 15 or 18 body sites up to three times, which have generated 5,177 microbial taxonomic profiles from 16S ribosomal RNA genes and over 3.5 terabases of metagenomic sequence so far. In parallel, approximately 800 reference strains isolated from the human body have been sequenced. Collectively, these data represent the largest resource describing the abundance and variety of the human microbiome, while providing a framework for current and future studies.

DOI10.1038/nature11209
Alternate JournalNature
PubMed ID22699610
PubMed Central IDPMC3377744
Grant ListDP2 OD001500 / OD / NIH HHS / United States
DP2OD001500 / OD / NIH HHS / United States
N01AI30071 / AI / NIAID NIH HHS / United States
N01AI30071 / AI / NIAID NIH HHS / United States
N01HG62088 / HG / NHGRI NIH HHS / United States
N01HG62088 / HG / NHGRI NIH HHS / United States
P30 DE020751 / DE / NIDCR NIH HHS / United States
P30DE020751 / DE / NIDCR NIH HHS / United States
R01 DE021574 / DE / NIDCR NIH HHS / United States
R01 HG004853 / HG / NHGRI NIH HHS / United States
R01 HG004856 / HG / NHGRI NIH HHS / United States
R01 HG004857 / HG / NHGRI NIH HHS / United States
R01 HG004872 / HG / NHGRI NIH HHS / United States
R01 HG004877 / HG / NHGRI NIH HHS / United States
R01 HG004885 / HG / NHGRI NIH HHS / United States
R01 HG004900 / HG / NHGRI NIH HHS / United States
R01 HG004906 / HG / NHGRI NIH HHS / United States
R01 HG004908 / HG / NHGRI NIH HHS / United States
R01 HG005171 / HG / NHGRI NIH HHS / United States
R01 HG005172 / HG / NHGRI NIH HHS / United States
R01 HG005969 / HG / NHGRI NIH HHS / United States
R01 HG005975 / HG / NHGRI NIH HHS / United States
R01DE021574 / DE / NIDCR NIH HHS / United States
R01HG00485 / HG / NHGRI NIH HHS / United States
R01HG004853 / HG / NHGRI NIH HHS / United States
R01HG004856 / HG / NHGRI NIH HHS / United States
R01HG004872 / HG / NHGRI NIH HHS / United States
R01HG004877 / HG / NHGRI NIH HHS / United States
R01HG004885 / HG / NHGRI NIH HHS / United States
R01HG004900 / HG / NHGRI NIH HHS / United States
R01HG004906 / HG / NHGRI NIH HHS / United States
R01HG004908 / HG / NHGRI NIH HHS / United States
R01HG005171 / HG / NHGRI NIH HHS / United States
R01HG005172 / HG / NHGRI NIH HHS / United States
R01HG005969 / HG / NHGRI NIH HHS / United States
R01HG005975 / HG / NHGRI NIH HHS / United States
R21 CA139193 / CA / NCI NIH HHS / United States
R21CA139193 / CA / NCI NIH HHS / United States
R21HG005811 / HG / NHGRI NIH HHS / United States
R37 DE016937 / DE / NIDCR NIH HHS / United States
RC1 DE020298 / DE / NIDCR NIH HHS / United States
RC1DE020298 / DE / NIDCR NIH HHS / United States
T32AI007528 / AI / NIAID NIH HHS / United States
U01 DE016937 / DE / NIDCR NIH HHS / United States
U01 HG004866 / HG / NHGRI NIH HHS / United States
U01DE016937 / DE / NIDCR NIH HHS / United States
U01HG004866 / HG / NHGRI NIH HHS / United States
U54 AI084844 / AI / NIAID NIH HHS / United States
U54 HG003067 / HG / NHGRI NIH HHS / United States
U54 HG003079 / HG / NHGRI NIH HHS / United States
U54 HG003273 / HG / NHGRI NIH HHS / United States
U54 HG004968 / HG / NHGRI NIH HHS / United States
U54 HG004969 / HG / NHGRI NIH HHS / United States
U54 HG004973 / HG / NHGRI NIH HHS / United States
U54AI084844 / AI / NIAID NIH HHS / United States
U54HG003067 / HG / NHGRI NIH HHS / United States
U54HG003079 / HG / NHGRI NIH HHS / United States
U54HG003273 / HG / NHGRI NIH HHS / United States
U54HG004968 / HG / NHGRI NIH HHS / United States
U54HG004969 / HG / NHGRI NIH HHS / United States
U54HG004973 / HG / NHGRI NIH HHS / United States
UH2 AI083263 / AI / NIAID NIH HHS / United States
UH2 AR057504 / AR / NIAMS NIH HHS / United States
UH2 DK083990 / DK / NIDDK NIH HHS / United States
UH2AI083263 / AI / NIAID NIH HHS / United States
UH2AR057504 / AR / NIAMS NIH HHS / United States
UH2AR057506 / AR / NIAMS NIH HHS / United States
UH2DK083990 / DK / NIDDK NIH HHS / United States
UH3 AI083263 / AI / NIAID NIH HHS / United States
UH3 DK083993 / DK / NIDDK NIH HHS / United States
UH3AI083263 / AI / NIAID NIH HHS / United States
UH3AR057504 / AR / NIAMS NIH HHS / United States
UH3DK083993 / DK / NIDDK NIH HHS / United States
/ / Howard Hughes Medical Institute / United States